Osmotic Properties of Protoplasts of Micrococcus lysodeikticus
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چکیده
منابع مشابه
Liberation and osmotic properties of the protoplasts of Micrococcus lysodeikticus and Sarcina lutea.
Stable protoplasts may be released from MiCrococcua Zysodeikticus and Sarcina Zutea by digestion of the cell-wall with lysozyme in sucrose or NaCl solutions having an osmotic pressure of some 25 atmospheres, but not in glycerol solutions of the same osmotic pressure. The stability of the protoplasts depends not only upon the depression of the water activity by the solute but upon an osmotic pre...
متن کاملBinding of bacitracin to cells and protoplasts of Micrococcus lysodeikticus.
Bacitracin was tritiated by the Wilzbach technique and purified by carboxymethylcellulose chromatography. Binding studies between [3H]bacitracin and Micrococcus lysodeikticus cells and protoplasts indicated that the cells or protoplasts were saturated at 2 X lo5 molecules per cell. This value correlates well with the number of CS5-isoprenyl pyrophosphate molecules found in the membrane and adds...
متن کاملImmunological properties of Micrococcus lysodeikticus membranes.
Membranes of Micrococcus lysodeikticus possess antigens which are distinct from other cellular components such as cytoplasm, ribosomes, and cell walls. Only a few (two to three) components are found when dissociated membranes are examined by immunodiffusion and immunoelectrophoresis techniques. Membranes treated with 0.3% sodium dodecyl sulfate, 0.3% Triton X-100, trypsin, phospholipase A or C,...
متن کاملInduction of lysogenic Micrococcus lysodeikticus.
Field, A. K. (Cornell University, Ithaca, N.Y.) and H. B. Naylor. Induction of lysogenic Micrococcus lysodeikticus. J. Bacteriol. 84:1129-1133. 1962.-Between 50 and 60% of log-phase cells of Micrococcus lysodeikticus strain ML 53-40, lysogenic for N5 bacteriophage, were induced by an optimal dose of ultraviolet light. Induction of dilute cell suspensions caused a subsequent 200- to 800-fold inc...
متن کاملMolecular properties of succinate dehydrogenase isolated from Micrococcus luteus (lysodeikticus).
Succinate dehydrogenase (EC 1.3.99.1) of Micrococcus luteus was selectively precipitated from Triton X-100-solubilized membranes by using specific antiserum. The precipitated enzyme contained equimolar amounts of four polypeptides with apparent molecular weights of 72,000, 30,000, 17,000, and 15,000. The 72,000 polypeptide possessed a covalently bound flavin prosthetic group and appeared to be ...
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ژورنال
عنوان ژورنال: Journal of General Microbiology
سال: 1959
ISSN: 0022-1287
DOI: 10.1099/00221287-20-2-321